Prism, available for both Windows and Mac computers, combines
scientific graphing, comprehensive curve fitting (nonlinear
regression), understandable statistics, and data organization.
GraphPad Prism was originally designed for experimental biologists
in medical schools and drug companies, especially those in
pharmacology and physiology. Prism is now used much more broadly by
all kinds of biologists, as well as social and physical scientists.
More than 200,000 scientists in over 110 countries rely on Prism to
analyze, graph and present their scientific data. It is also widely
used by undergraduate and graduate students.
regression is an important tool in analyzing data, but is often more
difficult than it needs to be. No other program simplifies curve
fitting like Prism. In fact, you can usually fit curves in a single
step. Just select an equation from the extensive list of commonly
used equations (or enter your own equation) and Prism does the rest
automatically -- fits the curve, displays the results as a table,
draws the curve on the graph, and interpolates unknown values.
Place data for multiple data sets side-by-side on an organized data
table, and Prism can fit them all the data sets at once. You can fit
the same model separately to each data set, use global nonlinear
regression to share parameter values among data sets, or fit
different models to different data sets.
Don't be fooled by the simplicity. Prism also gives you many
advanced fitting options. It can automatically interpolate unknown
values from a standard curve (i.e., to analyze RIA data), compare
the fits of two equations using an F test or Akaike's Information
Criterion (AIC), plot residuals, identify outliers, differentially
weight data points, test residuals for normality, and much more.
While it won't
replace a heavy-duty statistics program, Prism lets you easily
perform basic statistical tests commonly used by laboratory and
clinical researchers. Prism offers t tests, nonparametric
comparisons, one- and two-way ANOVA, analysis of contingency tables,
and survival analysis. Analysis choices are presented in clear
language that avoids unnecessary statistical jargon.
programs, Prism provides understandable statistical help when you
need it. Press "Learn" from any data analysis dialog and Prism's
online documentation will explain the principles of the analysis to
help you make appropriate choices. Once you've made your choices,
Prism presents the results on organized, easy-to-follow tables. The
Prism documentation goes beyond anything you would expect. More than
half of it is devoted to thorough explanations of basic statistics
and nonlinear curve fitting, to teach you what you need to know to
appropriately analyze your data.
completed the analysis, Prism’s unique analysis checklists help you
make sure you chose an analysis appropriate for your experimental
design, and that you understand the assumptions behind the analysis.
Retrace every analysis
really science unless you can document exactly how your data were
analyzed. WIth Prism this isn’t a problem. You’ll never wonder how
the results got there. Even if the work was done by someone else,
you can review (and change) all analysis choices, and see the
sequence of analyses (i.e. that the X values were transformed to
logarithms before the curve was fit). Similarly, you can easily
check if error bars represent the SD or SEM (or something else).
All parts of
your Prism project are linked. This means that when you fix a data
entry error, Prism automatically updates all results, graphs, and
layouts. Another advantage is that you can instantly analyze a
repeat experiment. After you’ve polished the analysis and graphing
steps with data from one experiment, you don’t have to repeat all
those steps. Prism provides several ways to recycle your work -- to
instantly analyze and graph a repeat experiment, without repeating
any tedious steps and without requiring any scripting or
Automatic error bars
If you computed
error values elsewhere, enter them into labeled (SD or SEM)
subcolumns. Prism’s analyses (i.e. t test, ANOVA, regression) will
take into account the SD (or SEM) and sample size.
A complete record of
project file can contain more than data, analyses and graphs. A
Prism file can be a complete record of your experiments. Each file
can contain any number of data tables, info pages, analysis results,
graphs, and page layouts. Data tables can contain not only the data
you analyze and graph, but also the data you decided to exclude,
which remain on the data table (in blue italics). Each info page can
store both unstructured notes and structured info constants such as
lot numbers, concentrations, notebook page numbers, etc. Graphs and
layouts can be embellished with text, lines, arrows, boxes, tables,
equations, pictures and more. Annotate any page with color-coded
floating notes, and highlight the names of pages you want to look at
again, or want someone else to review. Save Prism files on your
computer or network or save directly to LabArchives, a cloud-based
Ready to publish
Prism takes you
from raw data to a graph (or layout) ready for presentations,
posters or publications. A single click sends completed graphs or
layouts to PowerPoint or Word. Copy and paste works too, of course.
Export to TIFF, EPS, JPG, or PDF (and more), with all the options
you need to satisfy the requirements of scientific journals.